Editor-in-Chief:  T.B. Niewold
Associate Editors: 

Z. Dong
D. Duffy
B. Fife
E. M. Lennon
A. A. Sadighi Akha
L.E.B. Savio

The Journal of Immunological Methods is devoted to covering techniques for:

(1) Quantitating and detecting antibodies and/or antigens.
(2) Purifying immunoglobulins, cytokines and other molecules of the immune system.
(3) Isolating antigens and other substances important in immunological processes.
(4) Labelling antigens and antibodies.
(5) Localizing antigens and/or antibodies in tissues and cells.
(6) Detecting, and fractionating immunocompetent cells.
(7) Assaying for cellular immunity.
(8) Documenting cell-cell interactions.
(9) Initiating immunity and unresponsiveness.
(10) Transplanting tissues.
(11) Studying items closely related to immunity such as complement, reticuloendothelial system and others.
(12) Molecular techniques for studying immune cells and their receptors.
(13) Imaging of the immune system.
(14) Methods for production or their fragments in eukaryotic and prokaryotic cells.
(15) Computational methods including statistical and mathematical methods and computational models for analysis, visualization, and interpretation of results.

Submitted full length manuscripts should describe new methods of broad applicability to immunology and not simply the application of an established method – although papers describing such applications may be considered for publication as a short Technical Note. Review articles will also be published by the Journal of Immunological Methods, but these manuscripts are by invitation only.

JIM-sponsored Awards

Since 2020, JIM has sponsored the following three AMLI Abstract Awards on an annual basis: the Young Investigator Award, the Doctorate Award, and the Laboratory Scientist Award.

• Young Investigator Award – For applicants 35 years or younger.
  • 2024 – Qian Wang
  • 2023 – May Choi
  • 2022 – Amy Thees
  • 2021 – Hemant Raj Joshi
  • 2020 – Hannah Mathew

• Doctorate Award – For Ph.D., M.D., or equivalent degree holders.
  • 2024 – Ajay Grover
  • 2023 – Kyphuong Luong
  • 2022 – Hemant Raj Joshi
  • 2021 – Dharmendra Jain
  • 2020 – Thomas R. Haven

• Peter Maxim Memorial Clinical Laboratory Scientist Award – For Clinical Laboratory Scientists.
  • 2024 – Qian Wang
  • 2023 – MeLea Hedrick
  • 2022 – Patricia Merkel
  • 2021 – Jody Frinack
  • 2020 – Thomas B. Martins; LuAnn Rezavi

 

AMLI Members Involved with JIM

Editor-in-Chief

T.B. Niewold

Hospital for Special Surgery, New York, New York, United States of America

Associate Editors

A. A. Sadighi Akha – Mayo Clinic Minnesota Department of Laboratory Medicine and Pathology, Rochester, Minnesota, United States of America

Editorial Board

T.L. Bonfield – Cleveland, Ohio, United States
J. Bornhorst – Rochester, Minnesota, United States
J. C. Delgado – Salt Lake City, Utah, United States
B. Detrick – Baltimore, Maryland, United States
R.J. Harbeck – Denver, Colorado, United States of America
A. Kumanovics – Rochester, Minnesota, United States of America
G. Maine, – Royal Oak, Michigan, United States
J. R. Mills – Rochester, Minnesota, United States
Raymond Gerard/R.G. O’Gorman, – Los Angeles, California, United States
Lisa K. Peterson, – Salt Lake City, Utah, United States
J.L. Schmitz – Chapel Hill, North Carolina, United States of America

JIM publications authored by AMLI members since 2020

1. Profaizer T, Saadalla A, Nandakumar V. Validation for soluble C5b-9 detection and comparative analysis of three quantification methods. J Immunol Methods. 2025 Jun;541:113882. doi: 10.1016/j.jim.2025.113882. Epub 2025 May 22. PMID: 40412754.
2. Profaizer T, Elgort MG, Delgado JC. Development and laboratory validation of an electrochemiluminescence ELISA technique for measuring infliximab concentrations and anti-drug antibodies. J Immunol Methods. 2025 May;540:113865. doi: 10.1016/j.jim.2025.113865. Epub 2025 Apr 21. PMID: 40268228.
3. Bleesing J. The Fas-mediated apoptosis assay: From concept to clinical application. J Immunol Methods. 2025 Feb;537:113812. doi: 10.1016/j.jim.2025.113812. Epub 2025 Jan 26. PMID: 39875002.
4. Marsh RA, Bleesing JJ, Chiang SCC. Diagnostic testing for hemophagocytic lymphohistiocytosis. J Immunol Methods. 2025 Feb;537:113816. doi: 10.1016/j.jim.2025.113816. Epub 2025 Jan 22. PMID: 39855542.
5. Knight V. Phospho-flow cytometry assays for diagnostic use – A discussion of assay utility and assay development and validation challenges. J Immunol Methods. 2025 Feb;537:113818. doi: 10.1016/j.jim.2025.113818. Epub 2025 Jan 22. PMID: 39855543.
6. Lázár-Molnár E, Peterson LK. Clinical utility of the lymphocyte proliferation assay, an in vitro functional readout of the adaptive immune response. J Immunol Methods. 2025 Feb;537:113819. doi: 10.1016/j.jim.2025.113819. Epub 2025 Jan 22. PMID: 39855541.
7. Kumánovics A, Askar M, Sadighi Akha AA. Functional immunological assays. J Immunol Methods. 2025 Feb;537:113822. doi: 10.1016/j.jim.2025.113822. Epub 2025 Jan 31. PMID: 39892827.
8. Nandakumar V, Braun KMP, Willrich MAV. Challenges for complement functional assays in the clinical laboratory: From test validation to clinical interpretation. J Immunol Methods. 2025 Feb;537:113824. doi: 10.1016/j.jim.2025.113824. Epub 2025 Feb 4. PMID: 39914516.
9. Rountree W, Lynch HE, Denny TN, Sempowski GD, Macintyre AN. Sources of variability in Luminex bead-based cytokine assays: Evidence from twelve years of multi-site proficiency testing. J Immunol Methods. 2024 Aug;531:113699. doi: 10.1016/j.jim.2024.113699. Epub 2024 May 31. PMID: 38823575; PMCID: PMC11246216.
10. Elzieny M, Maine GN, Carey-Ballough RA, Sun Q. Discrepancies between two total IgE assays and difference in reference intervals in healthy adults. J Immunol Methods. 2024 Aug;531:113711. doi: 10.1016/j.jim.2024.113711. Epub 2024 Jun 13. PMID: 38878960.
11. Wills S, Chavez J, Grover A, Beck N, Romano M, Bauer C, Gerspach M, Schneider M, Valcour A. PD-BAT: A novel approach of pooling basophil donors for expansion of commercial laboratory testing of Chronic Spontaneous Urticaria. J Immunol Methods. 2024 Jun;529:113679. doi: 10.1016/j.jim.2024.113679. Epub 2024 Apr 27. PMID: 38679364.
12. Martins TB, Hill HR, Peterson LK. Specimen type validation and establishment of normal cytokine reference intervals in cerebrospinal fluid. J Immunol Methods. 2024 Jun;529:113681. doi: 10.1016/j.jim.2024.113681. Epub 2024 May 1. PMID: 38701879.
13. Luong K, Lozier BK, Novis CL, Smith TL, Zuromski LM, Peterson LK. Comparison of three methods for the detection of antibodies against muscle-specific kinase. J Immunol Methods. 2024 Mar;526:113627. doi: 10.1016/j.jim.2024.113627. Epub 2024 Feb 3. PMID: 38311009.
14. Sadighi Akha AA, Kumánovics A. Anti-cytokine autoantibodies and inborn errors of immunity. J Immunol Methods. 2022 Sep;508:113313. doi: 10.1016/j.jim.2022.113313. Epub 2022 Jul 9. PMID: 35817172.
15. Kumánovics A, Sadighi Akha AA. Flow cytometry for B-cell subset analysis in immunodeficiencies. J Immunol Methods. 2022 Oct;509:113327. doi: 10.1016/j.jim.2022.113327. Epub 2022 Aug 5. PMID: 35934071.
16. Peterson LK. Application of vaccine response in the evaluation of patients with suspected B-cell immunodeficiency: Assessment of responses and challenges with interpretation. J Immunol Methods. 2022 Nov;510:113350. doi: 10.1016/j.jim.2022.113350. Epub 2022 Sep 5. PMID: 36067869.
17. Nandiwada SL. Overview of human B-cell development and antibody deficiencies. J Immunol Methods. 2023 Aug;519:113485. doi: 10.1016/j.jim.2023.113485. Epub 2023 May 5. PMID: 37150477.
18. Knight V, Snyder MR. Editorial introduction for “B cell immunodeficiencies” Journal of Immunological Methods special edition. J Immunol Methods. 2023 Aug;519:113505. doi: 10.1016/j.jim.2023.113505. Epub 2023 Jun 7. PMID: 37290738.
19. Sims MD, Podolsky RH, Childers KL, Higgins B, Trueman J, Homayouni R, Voss DR, Berkiw-Scenna N, Keil H, Kennedy RH, Maine GN. Dried blood spots are a valid alternative to venipuncture for COVID-19 antibody testing. J Immunol Methods. 2023 Feb;513:113420. doi: 10.1016/j.jim.2022.113420. Epub 2022 Dec 31. PMID: 36596443; PMCID: PMC9804961.
20. Blincoe A, Labrosse R, Abraham RS. Acquired B-cell deficiency secondary to B-cell-depleting therapies. J Immunol Methods. 2022 Dec;511:113385. doi: 10.1016/j.jim.2022.113385. Epub 2022 Nov 11. PMID: 36372267.
21. Cornaby C, Giardina CK, Schmitz JL. Repeatability of QuantiFERON-TB gold plus testing utilizing microparticle chemiluminescence. J Immunol Methods. 2022 Oct;509:113340. doi: 10.1016/j.jim.2022.113340. Epub 2022 Aug 27. PMID: 36030829.
22. Van Buren K, Li Y, Zhong F, Ding Y, Puranik A, Loomis CA, Razavian N, Niewold TB. Artificial intelligence and deep learning to map immune cell types in inflamed human tissue. J Immunol Methods. 2022 Jun;505:113233. doi: 10.1016/j.jim.2022.113233. Epub 2022 Feb 4. PMID: 35131237.
23. Fatica EM, Larson BJ, Algeciras-Schimnich A, Bornhorst JA. Performance characteristics of the BRAHMS KRYPTOR automated squamous cell carcinoma antigen assay. J Immunol Methods. 2022 May;504:113257. doi: 10.1016/j.jim.2022.113257. Epub 2022 Mar 15. PMID: 35304118.
24. Maine GN, Krishnan SM, Walewski K, Trueman J, Sykes E, Sun Q. Clinical and analytical evaluation of the Abbott AdviseDx quantitative SARS-CoV-2 IgG assay and comparison with two other serological tests. J Immunol Methods. 2022 Apr;503:113243. doi: 10.1016/j.jim.2022.113243. Epub 2022 Feb 16. PMID: 35181288; PMCID: PMC8847080.
25. Huey L, Andersen G, Merkel PA, Morrison TE, McCarthy M, DomBourian MG, Annen K, Dawson ED, Rowlen KL, Knight V. Evaluation of a multiplexed coronavirus antigen array for detection of SARS-CoV-2 specific IgG in COVID-19 convalescent plasma. J Immunol Methods. 2021 Oct;497:113104. doi: 10.1016/j.jim.2021.113104. Epub 2021 Jul 22. PMID: 34303688; PMCID: PMC8297968.
26. Lozier BK, Haven TR, Tebo AE, Peterson LK. Performance evaluation of a radioimmunoprecipitation assay for the detection of N-type voltage-gated calcium channel antibodies. J Immunol Methods. 2021 Sep;496:113102. doi: 10.1016/j.jim.2021.113102. Epub 2021 Jul 21. PMID: 34298066.
27. Knight V, Askar MZ, Ntrivalas E, Nandiwada SL, Peterson LK, Tebo AE, Kadkhoda K, Schmitz JL, Naides SJ, Snyder MR, Sadighi Akha AA. Highlights of the 33rd annual scientific meeting of the Association of Medical Laboratory Immunologists (AMLI). J Immunol Methods. 2021 May;492:112994. doi: 10.1016/j.jim.2021.112994. Epub 2021 Feb 21. PMID: 33626382; PMCID: PMC7959703.
28. Willrich MAV, Ladwig PM, Martinez MA, Sridharan MR, Go RS, Murray DL; Complement Alternative Pathway Thrombotic Microangiopathy (CAP-TMA) Disease Oriented Group at the Mayo Clinic. Monitoring Ravulizumab effect on complement assays. J Immunol Methods. 2021 Mar;490:112944. doi: 10.1016/j.jim.2020.112944. Epub 2020 Dec 13. PMID: 33321132.
29. Lundgren MC, Sapkota S, Peterson DJ, Crosson JT. The antinuclear antibody dense fine speckled pattern and possible clinical associations: An indication of a proinflammatory microenvironment. J Immunol Methods. 2021 Jan;488:112904. doi: 10.1016/j.jim.2020.112904. Epub 2020 Oct 27. PMID: 33121975.
30. DomBourian MG, Annen K, Huey L, Andersen G, Merkel PA, Jung S, Dominguez SR, Knight V. Analysis of COVID-19 convalescent plasma for SARS-CoV-2 IgG using two commercial immunoassays. J Immunol Methods. 2020 Nov;486:112837. doi: 10.1016/j.jim.2020.112837. Epub 2020 Aug 20. PMID: 32828791; PMCID: PMC7438987.